Angiotensin Converting Enzyme-2, ACE2 from MilliporeSigma

Product Specs
ItemAngiotensin Converting Enzyme-2, ACE2
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberSAE0064
ApplicationsVirus Characterization

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Pineapple Lectin AcmJRL Binds SARS‐CoV‐2 Spike Protein in a Carbohydrate‐Dependent Fashion**ChemBioChemFebruary 1, 2023Joscha Meiers, Jan Dastbaz, Sebastian Adam, Sari Rasheed, Susanne H. Kirsch, Peter Meiser, Peter Gross, Rolf Müller, Alexander Titzgroups to obtain a final response of ∼6700 RU (2626 RU for Figure S6). The ACE2 receptor (Sigma‐Aldrich, SAE0064) was immobilised on a CM5 sensor chip using the same conditions and a final response of ∼990 RU was

(2) An albumin-angiotensin converting enzyme 2-based SARS-CoV-2 decoy with FcRn-driven half-life extensionActa BiomaterialiaSeptember 24, 2022Elisabeth Fuchs, Imke Rudnik-Jansen, Anders Dinesen, Denis Selnihhin, Ole Aalund Mandrup, Kader Thiam, Jørgen Kjems, Finn Skou Pedersen, Kenneth A. HowardBio-Layer Interferometry (BLI) using an Octet Red 96e system (Sartorius). Soluble ACE2 (Sigma Aldrich, #SAE0064) was used as a control. The biotinylated SARS-CoV-2 S1 protein was immobilised on Streptavidin Dip and

(3) Production and secretion of functional SARS-CoV-2 spike protein in Chlamydomonas reinhardtiiFrontiers in Plant ScienceSeptember 20, 2022Anna Maria Kiefer, Justus Niemeyer, Anna Probst, Gerhard Erkel, Michael Schrodaprotein binding assay to recombinant hACE2 Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL. 1 μg of hACE2 was mixed

(4) Evidence in favor of the essentiality of human cell membrane-bound ACE2 and against soluble ACE2 for SARS-CoV-2 infectivityCellMay 26, 2022Daniel Batlle et al.also used a paired t test. Yeung et al. (2021) used soluble ACE2 protein purchased from Sigma (Cat#SAE0064) which, like our protein (see Supplemental methods), has amino acids 18–740 and dimerizes via its collectrin-like

(5) Potent neutralization of SARS-CoV-2 variants of concern by an antibody with an uncommon genetic signature and structural mode of spike recognitionCell reportsOctober 5, 2021Kramer KJ, Johnson NV, Shiakolas AR, Suryadevara N, Periasamy S, Raju N, Williams JK, Wrapp D, Zost SJ, Walker LM, et al.,Sino Biological,Cat#40591-V49H HCoV-229E S,Sino Biological,Cat#40605-V08B HCoV-NL63 S,Sino Biological,Cat#40604-V08B ACE2,Sigma Aldrich,SAE0064-5UG Streptavidin HRP,Thermo Fisher Scientific,Cat#ENN100 Streptavidin R-phycoerythrin (SA-PE),Invitrogen,Cat#S866 Carboxyfluorescein

(6) Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individualsCell reportsAugust 24, 2021Chen EC, Gilchuk P, Zost SJ, Suryadevara N, Winkler ES, Cabel CR, Binshtein E, Chen RE, Sutton RE, Rodriguez J, et al.Alexa Fluor 647,Invitrogen,Cat#: A-21445 "Angiotensin Converting Enzyme-2, ACE-2",Sigma-Aldrich,Cat#: SAE0064 Monoclonal ANTI-FLAG M2-Peroxidase HRP,Sigma-Aldrich,Cat#: A8592 Anti-S2 antibody 1A9,Genetex,Cat#:

(7) Nonalcoholic Fatty Liver Disease Risk Factors Affect Liver-Related Outcomes After Direct-Acting Antiviral Treatment for Hepatitis CDigestive Diseases and SciencesJuly 1, 2021Jihane N. Benhammou, Andrew M. Moon, Joseph R. Pisegna, Feng Su, Philip Vutien, Cynthia A. Moylan, George N. Ioannousaturate the antigen. Then, a mixture of recombinant hACE2 with a FLAG tag (Sigma-Aldrich cat. no. SAE0064), a rat anti-FLAG AF 647 antibody at a 1:50 dilution (BioLegend clone L5, cat. no. 637315, Lot B265929)

(8) Cross-reactive coronavirus antibodies with diverse epitope specificities and Fc effector functionsCell reports. MedicineJune 15, 2021Shiakolas AR, Kramer KJ, Wrapp D, Richardson SI, Schäfer A, Wall S, Wang N, Janowska K, Pilewski KA, Venkat R, et al.RBD,Sino Biological,Cat #40150-V08B2 SARS-CoV-2 NTD,Sino Biological,Cat#40591-V49H ACE2,Sigma Aldrich,SAE0064-5UG SARS-CoV-2 S HexaPro N165A,This paper,N/A SARS-CoV-2 S HexaPro N709A,This paper,N/A SARS-CoV-2

(9) Soluble ACE2-mediated cell entry of SARS-CoV-2 via interaction with proteins related to the renin-angiotensin systemCellApril 15, 2021Yeung ML, Teng JLL, Jia L, Zhang C, Huang C, Cai JP, Zhou R, Chan KH, Zhao H, Zhu L, et al.Bafilomycin A1,Sigma-Aldrich,Cat#SML1661 "Angiotensin Converting Enzyme-2, ACE2 (Human recombinant, expressed in HEK293 cells)",Sigma-Aldrich,Cat#SAE0064 "SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)",Sino Biological,Cat#40589-V08B1 Vasopressin,Origene,Cat#TP316816 ,, Deposited

(10) Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike proteinNature medicineSeptember 1, 2020Zost SJ, Gilchuk P, Chen RE, Case JB, Reidy JX, Trivette A, Nargi RS, Sutton RE, Suryadevara N, Chen EC, et al.saturate the antigen. Then, a mixture of recombinant hACE2 with a FLAG tag (Sigma-Aldrich cat. no. SAE0064), a rat anti-FLAG AF 647 antibody at a 1:50 dilution (BioLegend clone L5, cat. no. 637315, Lot B265929)

(11) Potently neutralizing and protective human antibodies against SARS-CoV-2NatureAugust 1, 2020Zost SJ, Gilchuk P, Case JB, Binshtein E, Chen RE, Nkolola JP, Schäfer A, Reidy JX, Trivette A, Nargi RS, et al.s, the biosensor tips then were immersed into the human ACE2 receptor (20 μg ml−1) (Sigma-Aldrich, SAE0064) for 300 s. The maximum binding of human ACE2 was normalized to a buffer-only control. Per cent binding

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Angiotensin Converting Enzyme-2, ACE2 from MilliporeSigma

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Angiotensin Converting Enzyme-2, ACE2 from MilliporeSigma

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